Summary:
CynX is a putative cyanate transporter. CynX is a member of the major facilitator superfamily (MFS) of transporters [ Pao98 ], and the cynX gene has been shown to be part of a cyanate-induced operon with cynS and cynT, encoding cyanase and carbonic anhydrase, respectively [ Guilloton93 , Sung88 ]. However, no phenotype has been observed for a knockout mutation in cynX [ Guilloton93 ].

Gene Citations: [ Anderson90 , Lamblin94 , Sung88 ]

Locations: inner membrane

Molecular Weight of Polypeptide: 41.361 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0001175 , CGSC:31261 , EchoBASE:EB0174 , EcoGene:EG10177 , EcoliHub:cynX , EcoliWiki:b0341 , ModBase:P17583 , OU-Microarray:b0341 , RefSeq:NP_414875 , RegulonDB:EG10177 , UniProt:P17583

Relationship Links: Pfam:IN-FAMILY:PF07690

GO Terms:

Biological Process:	GO:0006805 - xenobiotic metabolic process GO:0006810 - transport [ GOA00 , GOA01 ] GO:0009440 - cyanate catabolic process GO:0055085 - transmembrane transport [ GOA01 ]
Molecular Function:	GO:0005215 - transporter activity [ GOA01 ] GO:0015292 - uniporter activity
Cellular Component:	GO:0005886 - plasma membrane [ GOA00 ] GO:0016020 - membrane [ GOA00 ] GO:0016021 - integral to membrane [ GOA07 , GOA00 , GOA01 ]

Enzymatic reaction of: CynX cyanate MFS transporter

Synonyms: Transport of cyanate

Sequence Features

Feature Class	Location	Citations	Comment
Transmembrane-Region	3 -> 23	[ UniProt09 ]
Sequence-Conflict	27	[ UniProt09 ]	UniProt: (in Ref. 1; AAA23627); Non-Experimental Qualifier: variant;
Sequence-Conflict	27	[ Sung88 , UniProt09a ]	UniProt: (in Ref. 1; AAA23627);
Transmembrane-Region	34 -> 54	[ UniProt09 ]
Transmembrane-Region	68 -> 88	[ UniProt09 ]
Transmembrane-Region	89 -> 109	[ UniProt09 ]
Transmembrane-Region	122 -> 142	[ UniProt09 ]
Transmembrane-Region	153 -> 173	[ UniProt09 ]
Transmembrane-Region	204 -> 224	[ UniProt09 ]
Transmembrane-Region	235 -> 255	[ UniProt09 ]
Transmembrane-Region	263 -> 283	[ UniProt09 ]
Transmembrane-Region	287 -> 307	[ UniProt09 ]
Transmembrane-Region	322 -> 342	[ UniProt09 ]
Sequence-Conflict	336 -> 384	[ UniProt09 ]	UniProt: (in Ref. 1); Non-Experimental Qualifier: variant;
Sequence-Conflict	336 -> 384	[ Sung88 , UniProt09a ]	UniProt: (in Ref. 1);
Transmembrane-Region	354 -> 374	[ UniProt09 ]

Gene Local Context (not to scale):

Transcription Units:

Notes:

• Key to regulator colors: Green: activator; Magenta: inhibitor; Brown: dual; Gray: unspecified.
• A dashed baseline indicates that there is no high-quality evidence to confirm the extent of this transcription unit.
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• Click above or below gene(s) to navigate to detailed transcription unit display.
• Click on a binding site (if any) to navigate to the display for its transcription factor.
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History:
10/20/97 Gene b0341 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10177; confirmed by SwissProt match.

References

Anderson90: Anderson PM, Sung YC, Fuchs JA (1990). "The cyanase operon and cyanate metabolism." FEMS Microbiol Rev 1990;7(3-4);247-52. PMID: 2094285

GOA00: GOA (2000). "Gene Ontology annotation based on Swiss-Prot keyword mapping."

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA07: GOA, UniProt (2007). "Gene Ontology annotation based on Swiss-Prot Subcellular Location vocabulary mapping."

Guilloton93: Guilloton MB, Lamblin AF, Kozliak EI, Gerami-Nejad M, Tu C, Silverman D, Anderson PM, Fuchs JA (1993). "A physiological role for cyanate-induced carbonic anhydrase in Escherichia coli." J Bacteriol 1993;175(5);1443-51. PMID: 8444806

Lamblin94: Lamblin AF, Fuchs JA (1994). "Functional analysis of the Escherichia coli K-12 cyn operon transcriptional regulation." J Bacteriol 176(21);6613-22. PMID: 7961413

Pao98: Pao SS, Paulsen IT, Saier MH (1998). "Major facilitator superfamily." Microbiol Mol Biol Rev 1998;62(1);1-34. PMID: 9529885

Sung88: Sung YC, Fuchs JA (1988). "Characterization of the cyn operon in Escherichia coli K12." J Biol Chem 263(29);14769-75. PMID: 3049588

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt09a: UniProt Consortium (2009). "UniProt version 15.6 released on 28-JUL-09." Database.